Scotopic Critical Flicker Fusion in Preclinical AMD

Steven Nusinowitz, PhD
Jules Stein Eye Institute, University of California, Los Angeles (Los Angeles, CA)

Co-Principal Investigators

Michael Gorin, MD, PhD
Jules Stein Eye Institute (Los Angeles, CA)
Year Awarded:
2015
Grant Duration:
July 1, 2015 to June 30, 2018
Disease:
Macular Degeneration
Award Amount:
$160,000
Grant Reference ID:
M2015295
Award Type:
Standard
Award Region:
US Southwestern

This grant is made possible in part by a bequest from the Anne E. Greene Trust.

Steven Nusinowitz, PhD

Scotopic Critical Flicker Fusion in Preclinical AMD

Summary

If we are going to be able to treat people who are at risk of developing age-related macular degeneration (AMD), we need to be able to identify those people who would benefit most from a preventive treatment before they demonstrate classic signs of disease. Genetic testing alone is not sufficient to know who will develop AMD because many people who have high genetic susceptibility for AMD do not always develop the condition. In this project we are testing the possibility that our predictive models will be improved by introducing a novel method to evaluate retinal function.  We are testing the possibility that an early indication of visual impairment in people who do not yet have classic signs of AMD, is the impairment of the retina, particularly the cells that detect light at low levels, to discriminate flickering lights. We are developing a simple test for this purpose which we can use to measure function at multiple locations in the retina. We will determine whether this test can be used to help predict those who will develop AMD and/or to distinguish between different forms of AMD and their progression rates.

Details

The main goal of this research project is to develop and test a novel method of evaluating retinal function in patients who are risk of developing AMD, but who have not yet developed signs of the disease. We have identified an “at risk” population based on family history and their genetic profiles. However, while genetic testing can identify individuals with elevated or reduced risk for developing AMD, genetic testing alone is insufficient in discriminating those who will go on to develop AMD and progress to severe disease and vision loss. In this project, we are testing the hypothesis that genetic risk profiles, combined with our novel functional measures, will identify individuals at the earliest stages of AMD (ideally before there are visible retinal changes) who would benefit from early preventive therapies.

The functional measure that we are developing tests the ability of the retinal to distinguish discrete flashes of light over time. As an example, imagine a strobe light repeatedly flashing on and off. When the light flashes are presented intermittently at a relatively low flash rate, the perception of discrete flashes of light, best described as a flickering light, is clearly apparent. However, as the flash presentation rate is increased, an ambiguous perception of flicker is present, until eventually, at high presentation rates, the perception of discrete flashes of light is lost and the light appears to be on constantly. The phenomenon of flicker disappearance at high flash rates is called “flicker fusion.”

In the first aim, my laboratory will develop the instrumentation to evaluate retinal “flicker fusion” at multiple retinal locations that is mediated by our night vision system. While there have been numerous studies of flicker fusion mediated by our day vision system, the extension of such studies to night vision is unique, and since there are many studies that have shown night vision abnormalities even in early AMD, we expect that this unique methodology will provide evidence of early functional abnormalities in those who are at risk of developing AMD.  In the second aim, we will validate this methodology by studying how well it discriminates those with high AMD genetic risk profiles from those with low AMD risk profiles, but who do not yet have evidence of retinal pathology. In the third aim we will test the association of flicker fusion abnormalities with structural changes in the retina.

Therapy always comes at a cost, as well as with a benefit. Currently all of the approved treatments for AMD are focused on managing the end-stage complications of AMD. To be optimally effective, one needs to know who to treat, when to treat, and whether or not the treatment is having a desired effect without having to wait for an end-stage outcome. This study evaluates a novel functional biomarker that may prove to be a sensitive indicator of retinal dysfunction in pre-clinical AMD, which could also be a useful measure to reflect a response to preventive therapy.

About the Researcher

My primary research interest is the study of the sites and mechanisms of disease action in inherited eye diseases, including retinitis pigmentosa, AMD, and Stargardt disease. Using electrophysiological and psychophysical techniques, in my clinical laboratory I have been evaluating patients with diseases of the retina and known genetic abnormalities to better understand how specific gene mutations result in the wide spectrum of disease expression. In addition, we use these same techniques to study the natural history of disease in humans and to evaluate therapeutic interventions as they emerge. In my animal laboratory, we study mice with retinal disease in order to identify new candidate genes that may be involved in human disease. In addition, since the underlying biological failures in ocular disease are frequently not known, particularly in those visual disorders that have heterogeneous genetic etiologies, another goal of ours is to gain an understanding of the site and underlying mechanism of disease action in humans by studying the patterns of responses obtained from mice in which the disruption of different cells or pathways in the eye are specifically targeted by genetic manipulation. By comparing the patterns of electrophysiological responses in human disease with the patterns of responses from rodents with targeted cellular disruption, we are able to test hypotheses about the underlying pathophysiology in human disease, thus furnishing a mechanism for the development of specific diagnostic tools that are sufficiently sensitive for early detection of disease. Our research is also directed towards finding new ways to assess therapeutic intervention, and towards achieving earlier and better diagnosis of clinical disease.



"We have seen many patients over the years with macular degeneration and other retinal diseases who have asked whether we could have done something to stop or reverse their disease progress, had we detected their disease earlier. The answer to this question requires that we reach back into preclinical stages of disease, and that in turns depends on the development of sensitive measures, whether they involve structural or functional changes to the retina, to detect disease before classic signs of disease appear. We are not there yet, but the project which BrightFocus is funding is an essential effort in this direction.  It is a high-risk project, but the payoff could be enormous if our findings are fruitful. We are very grateful to BrightFocus and its donors for providing the means to carry out this important project."

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