A Novel Negative Immune Regulator to Control Wet AMD by Altering Immune-Vascular Crosstalk in the Eye

Ye Sun, MD, PhD
Children's Hospital Boston, Harvard Medical School (Boston, MA)
Year Awarded:
2019
Grant Duration:
July 1, 2019 to June 30, 2021
Disease:
Macular Degeneration
Award Amount:
$200,000
Grant Reference ID:
M2019114
Award Type:
Standard
Award Region:
US Northeastern
ye sun

A Negative Immune Regulator Controls Wet AMD

Summary

Abnormal blood vessel growth is a major cause of irreversible vision loss in age-related macular degeneration (AMD) patients. Currently there is no effective treatment to prevent or slow the blood vessel proliferation in wet AMD patients. This work aims to investigate a novel negative-immune regulator that may suppress inflammation-induced abnormal vessel growth in AMD by altering immune-vascular crosstalk. Novel activators of this immune regulator will be evaluated in a pre-clinical animal model of AMD to determine if treatment is effective in preventing or slowing development of AMD-like pathologies, and findings from this work will identify new treatments to prevent or treat wet AMD.

Details

Our studies aim to determine the root causes of new blood vessel formation (neovascularization) in wet AMD by targeting an immune regulator to modulate immune-vascular crosstalk using both genetic and pharmacological approaches. Abnormal new blood vessels lead to neuronal cell death and eventually cause blindness, however current treatments to block new blood vessels are only effective in ~50% of wet AMD patients and cause serious side effects that affect the health of normal blood vessels and retinal nerve cells. Understanding the primary drivers of neovascularization is critical and urgent. Our goal is to determine the drivers and identify new, effective, and safe treatments to prevent and treat wet AMD.

Choroidal neovascularization affects ~10% of AMD patients but accounts for up to 90% of vision loss associated with AMD. Immune-vascular crosstalk is a critical pathogenetic pathway in AMD. We will address the fundamental problems of immune dysfunction and inflammatory changes that lead to choroidal neovascularization development. Immune cells, especially myeloid cells, play a key role in linking innate and adaptive immunity, primarily through antigen presentation and recruitment of adaptive immune cells. Choroidal circulation brings to the eye a large number of immune cells, specifically those of myeloid origin, which play important roles in retinal and choroidal vascular pathology. In response to chronic insults, such as those occurring in AMD, myeloid cells become activated and release inflammatory mediators (such as growth factors, matrix metalloproteinases, interleukins, and chemokines) that stimulate ocular neovascularization. A number of chemokines attract immune cells to invade and infiltrate ocular tissues and furthermore stimulate the secretion of more trafficking molecules utilized by migrating immune cells. Increasing evidence shows that inflammatory changes in immune cells influence pathological angiogenesis, but the specifics are still unknown as to how immune cells strongly influence pathological vascular formation. Therefore, analyzing inflammatory mediators and the degree of myeloid cell activation and infiltration around choroidal neovascularization is necessary to understand how the innate inflammatory response contributes to choroidal neovascularization onset and progression. There is a lack of effective approaches to control immune/inflammation, needed to suppress choroidal neovascularization.  We aim to fill this gap by investigating a novel immune regulator that can control immune-vascular crosstalk and that may be targeted pharmacologically to suppress choroidal neovascularization in AMD. Our study is innovative in that it applies novel approaches (activation of a unique immunoregulator in immune cells) to suppress choroidal neovascularization, to prove the concept that pathological choroidal neovascularization formation is controlled by immune cells. The contribution of our research is expected to make conceptual advances on how inflammatory factor in immune cells controls pathological blood vessel growth via modulation of immune-vascular crosstalk. This impact of our studies will be significant because it will provide a novel target for developing therapeutic strategies that have broad translational importance in the prevention and treatment of AMD. For translation to the clinic, we will evaluate pharmacological activators of immune regulator in the AMD mouse model for new strategies with which to treat and prevent neovascular AMD. Successful completion of the aims will broaden the field of retinal vascular biology research to include modulation of immune-vascular crosstalk via a novel immune regulator to prevent pathological blood vessel growth.

About the Researcher

Ye Sun, MD, PhD, is an Instructor of Ophthalmology at Boston Children’s Hospital and Harvard Medical School. Dr. Sun completed her PhD in Molecular Biology at Jilin University in China and completed her postdoctoral training at Boston Children’s Hospital and Harvard Medical School. Dr. Sun’s research interests focus on the understanding the roles of neuroinflammation, immune-vascular crosstalk, and neurovascular interaction in the development of eye disorders (including AMD, retinopathy of prematurity, and diabetic retinopathy) using genetically modified mice combined with disease mouse models and developing effective ways to treat or prevent vision loss. In particular, her recent research focuses on the mechanisms of immune-vascular crosstalk to identify the root causes of neovascularization, aimed at developing therapeutic treatments for wet AMD.

Personal Story

As time goes by, the relatives and friends around us are getting old, and more and more loved ones are suffering from vision loss due to AMD. They cannot read, watch TV, or drive; in the future AMD may affect my own vision as well. We have no way to stop getting old, but I hope that one day we can find a way to stop AMD. The immune system helps protect the body, but as we age, the immune system does not work as well. My lab is very appreciative of and excited to use the funds provided by BrightFocus Foundation—to understand the relationship between immune system and AMD during aging, to work on the master regulators of immune system that control AMD using disease mouse models, and to help people with AMD. I am deeply grateful to all BrightFocus donors who so generously support the work of those trying to find cures for this disease

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