Exosomal microRNA from the RPE
Recent studies suggest that not only are there problems with the RPE, but that the choroidal blood vessels underneath the RPE also degenerate early in AMD. The RPE and the choroidal endothelial cells are separated by a thin barrier known as Bruch's membrane. It is unclear what signals are exchanged between the two types of cells and how their normal communication is interrupted during early AMD. Preliminary work completed by this research group has shown that microvesicles released from the RPE contain various small RNA fragments. The proposed studies will further examine how stress conditions related to AMD may change the content of these RPE microvesicles that, in turn, may alter functions of the choroidal endothelial cells.
This proposal will use various innovative approaches, such as a new mouse engineered to have AMD and a technique called high throughput gene array. Cai is studying a mechanism that accounts for the early dysfunction of RPE and choroid—perhaps chronic oxidative injury is a major factor of RPE degeneration and dry AMD. The results from this research could lead to a new AMD treatment with emphasis on strengthening the protection and repair mechanisms of the RPE. Hopefully, this can be achieved by target-based nutritional intervention combined with a healthy lifestyle.
First published on: July 10, 2012
Last modified on: May 31, 2012