Proteomic differences in normal and glaucomatous optic nerve

Sanjoy Bhattacharya, PhD
Cleveland Clinic Foundation (Cleveland, OH)
Year Awarded:
2005
Grant Duration:
April 1, 2005 to March 31, 2007
Disease:
Glaucoma
Award Amount:
$90,000
Grant Reference ID:
G2005047
Award Type:
Standard
Award Region:
US Midwestern

Proteomic differences in normal and glaucomatous optic nerve

Details


Dr. Bhattacharya believes there are intrinsic differences in protein composition in the optic nerve that put the individuals at increased risk to glaucoma and that protein modifications leads to progression of the disease. The specific aim of this project is to determine the differential protein profile between a normal and glaucomatous optic nerve and to confirm the identity of unique glaucoma-associated proteins. The identification of proteins and protein modifications may serve as markers for individuals predisposed to an increased risk of developing glaucoma. Such information will provide insight into the causes of glaucoma and lead to identification of possible drug targets for glaucoma therapy.

Publications

Bhattacharya, S.K., Crabb, J.S., Gu, X., and Crabb, J.W. (2006) Proteomics implicates peptidyl arginine deiminase 2 and optic nerve citrullination in glaucoma pathogenesis. Invest Ophthalmol Vis Sci. 47(6):2508-2514.  

Bhattacharya, S.K., Crabb, J.S., Annangudi, S.P., West, K.A., Gu, X., Sun, J., Bonilha, V.L., Smejkal, G., Shadrach, K., Hollyfield, J.G., and Crabb, J.W. (2005) Optic nerve fractionation for proteomics. In: SEPARATION METHODS IN PROTEOMICS (eds, GB Smejkal and A Lazarev) Marcel Dekker, New York. [Book Chapter] Bhattacharya, S.K., Rockwood, E.J., Smith, S.D., Bonilha, V.L., Crabb, J.S., Kuchtey, R.W., Robertson, N.G., Peachey, N.S., Morton, C.C., and Crabb, J.W. (2005) Proteomics reveals Cochlin deposits associated with glaucomatous trabecular meshwork. J Biol Chem. 280(7):6080-6084.  

Bonilha, V.L., Bhattacharya, S.K., West, K.A., Sun, J., Crabb, J.W., Rayborn, M.E., and Hollyfield, J.G. (2004) Proteomic characterization of isolated retinal pigment epithelium microvilli. Mol Cell Proteomics. 3:1119-1127.  

Bhattacharya SK. Retinal deimination in aging and disease. IUBMB Life. 2009 May;61(5):504-9.  

Algeciras ME, Takahara H, Bhattacharya SK. Mechanical stretching elevates peptidyl arginine deiminase 2 expression in astrocytes. Curr Eye Res. 2008 Nov;33(11):994-1001.  

Bhattacharya SK, Sinicrope B, Rayborn ME, Hollyfield JG, Bonilha VL. Age-related reduction in retinal deimination levels in the F344BN rat. Aging Cell. 2008 Jun;7(3):441-4. Epub 2008 Jan 31.  

Algeciras, M. E., and Bhattacharya, S. K. (2007) Targeting optic nerve citrullination in glaucoma: a role for peptidylarginine deiminase 2 (PAD2) inhibitors. Drug of the Future 37 (11), 1-7.
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