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Gene Identified In Dogs With Glaucoma May Offer Hope For Future Therapies In Humans

February 18, 2011


Beagles born with an inherited form of glaucoma, similar to primary open angle glaucoma in humans, have a change or mutation in the “spelling” of a gene called ADAMTS10. This gene is expressed primarily in the trabecular meshwork, the filter for “draining” aqueous humor in the eye. The resulting change to the ADAMTS10 protein due to the mutation is thought to cause a blockage in aqueous humor drainage and an increase in eye pressure, leading to glaucoma in these dogs.


So far, three genes have been found associated with risk of glaucoma in humans, but there are still many forms of glaucoma for which no genes have been found. This dog gene is a strong candidate for testing in humans, in particular since this is the third ADAM family gene found associated with eye disease. If this gene does turn out to be a major contributing factor, then gene therapy treatments could become a possibility for many people with this devastating disease.

Co-author Jonathan Haines, Ph.D., from Vanderbilt University in Nashville, TN, is currently funded by the Macular Degeneration Research program of the BrightFocus Foundation.

For more details, please read the press release below, adapted from the Vanderbilt University Medical Center


Glaucoma—a leading cause of vision loss and blindness worldwide—runs in families. A team of investigators from Vanderbilt University and the University of Florida has identified a new candidate gene for the most common form of the eye disorder, primary open-angle glaucoma (POAG).

The findings, reported February 17 in the open-access journal PLoS Genetics, offer novel insights into glaucoma pathology and could lead to targeted treatment strategies.

Elevated pressure inside the eye is a strong risk factor for POAG. Pressure increases because of increased resistance to the flow of aqueous humor out of the eye's front chamber (between the cornea and iris). Current treatments for POAG attempt to reduce intraocular pressure by reducing aqueous humor production or by surgically providing a clear "drain."

"It has been known for decades that the reason the pressure goes up in POAG is because the outflow pathway for aqueous humor is not working," said Rachel Kuchtey, M.D., Ph.D., assistant professor of Ophthalmology and Visual Sciences and principal investigator of the current studies.

"It seems kind of simple—there's a decrease in the rate of aqueous humor flowing out of the eye," said John Kuchtey, Ph.D., research instructor in Ophthalmology and Visual Sciences and first author of the paper. "But the basic mechanisms of aqueous humor outflow at the cellular and molecular level—and how they are disrupted in glaucoma—are not understood. It's a long-standing puzzle in ophthalmology."

So far, three genes have been associated with human glaucoma, but they account for only a small fraction of cases and have not shed much light on the disease process. The Vanderbilt investigators turned to a model with simpler genetics—a canine model of the disease.

Forty years ago, Kirk Gelatt, V.M.D., from the UF College of Veterinary Medicine, came across a litter of beagles that had a high incidence of glaucoma. The finding suggested to Gelatt that the disease was inherited, and he established a colony of POAG-affected beagles to study treatments for the disease.

In affected beagles, intraocular pressure begins to increase at 8 to 16 months of age, due to increased resistance to aqueous humor outflow. The clinical course of the disease "absolutely resembles human glaucoma," Rachel Kuchtey said.

The beagles are the only naturally occurring animal model for human POAG.

The Vanderbilt investigators used blood samples to search for genes associated with POAG. They first narrowed in on a certain spot (locus) on canine chromosome 20, which matched part of human chromosome 19. Previous studies had associated the human region with intraocular pressure, a good sign that they were on the right track, John Kuchtey said.

Sequencing of the entire canine locus—over 4 million "letters" of DNA—revealed that a gene called ADAMTS10 was the strongest disease-associated candidate. POAG-affected dogs have a single mutation in the gene, which encodes a protein involved in processing the extracellular matrix (ECM), the connective and structural support tissue around cells.

"There is a lot of evidence that proteoglycans (molecules in the ECM) and matrix remodeling might have something to do with aqueous outflow resistance, and so this gene supports that line of investigation," John Kuchtey said.

The researchers also demonstrated that the gene is highly expressed in the trabecular meshwork—the specialized filtration tissue through which aqueous humor passes, another supportive piece of evidence that it may have a role in regulating aqueous humor outflow.

The investigators are currently exploring ADAMTS10's normal biological functions, and they have studies in progress to examine whether the human ADAMTS10 gene is mutated in human glaucoma. They are hopeful that understanding this gene will open therapeutic possibilities for glaucoma.

"Right now we know that aqueous outflow is impaired in POAG, but we have no way to fix it because we don't understand how that normally works and what the pathology is in POAG," Rachel Kuchtey said.

"If this gene truly plays a role in aqueous outflow regulation, we can begin to look at it—or its molecular partners—as targets for treatments."
Gene therapy to rescue a defect might also be a possibility. Gene therapy for an inherited form of childhood blindness was first validated in dogs and is now in trial in humans.

View all news updates for glaucoma

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Some of the content in this section is adapted from other sources, which are clearly identified within each individual item of information.

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