Amyloid precursor protein in synapse regulation

Hui Zheng, PhD
Baylor College of Medicine (Houston, TX)
Year Awarded:
2008
Grant Duration:
April 1, 2008 to August 31, 2011
Disease:
Alzheimer's Disease
Award Amount:
$400,000
Grant Reference ID:
A2008052
Award Type:
Standard
Award Region:
US Southern
Hui Zheng, PhD

Role of APP in synaptogenesis

Summary

Synapse dysfunction triggered by ABeta is central to Alzheimer's disease progression. While ABeta is derived from processing of APP, a physiological function for APP has not been clearly defined. This project will provide fundamental insights into Alzheimer's disease by providing a better understanding of synaptic roles for APP and of the mechanisms by which AB generation interferes with normal APP function.

Details

Beta-amyloid plaque is the hallmark of Alzheimer's disease (AD). The plaque is formed from the amyloid precursor protein (APP). Genetic mutations in APP lead to genetically inheritable forms of AD. Thus, APP's role is essential in AD pathogenesis. In addition to the plaque pathology, synapse dysfunction is believed to directly contribute to dementia. However, the link between APP and synaptic activity is poorly understood. Because it is difficult to know what happens in human brains during the disease progression, my laboratory uses mice as a model system to learn the human disease. Specifically, we are looking to understand the function of APP, and what goes wrong in familial cases that lead to AD using mice that either delete APP or express the mutant form of APP. We found that APP directly regulate synapse formation and we want to know how it works and whether the amyloid affect this activity. Our studies will allow understanding how APP functions in synaptic activity, what happens when APP is mutated, and what can we do to enhance the neuronal activity. The latter has the potential to discover novel therapeutic targets for AD.

Research Updates

Beta-amyloid plaque deposits in the brain are one of the hallmarks of Alzheimer's disease (AD). A plaque is formed from the bunching of amyloid precursor protein (APP). Genetic mutations in the APP gene lead to familial or early-onset forms of AD, with creation of plaques. Thus, APP's role is central in AD pathogenesis. In addition to the plaque deposits, loss of synapse activity, through which neurons communicate, is believed to directly contribute to dementia. However, the link between APP dysfunction and synaptic activity is poorly understood. My laboratory uses mouse models of the human forms of Alzheimer's disease to understand the function of APP in neurons and the changes caused by AD mutations.

In mouse models with either no APP expression or with expression of only the mutant form of APP and neurons derived from both sets of mice, we found that APP may serve as bridging molecules for the formation of the synapse. When the synapse isn't properly formed, due to the lack of proper APP function, then the synapse activity is low or absent. Our results will allow us to understand how this may be happening, what happens when APP is mutated or when beta-amyloid plaques are present, and what we can do to counteract this effect to enhance the synaptic activity and neural communication. The results of this study will be the basis for future discoveries of new therapies for increasing the synaptic activity found in AD, with the goal to improve cognitive function.

Publications

Wang, Z., Wang, B., Yang, L., Guo, Q., Aithmitti, N., Songyang, Z. and Zheng, H. (2009) Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J. Neurosci., 29:10788-10801.

Yang, L., Wang, Z., Wang, B., Justice, N., and Zheng, H. (2009) Amyloid precursor protein regulates Cav1.2 L-type calcium channel levels and function to influence GABAergic short-term plasticity. J. Neurosci., 29: 15660-15668.

Li, H., Wang, B., Wang, Z., Guo, Q., Tabuchi, K., Hammer, R., Sudhof, T., and Zheng, H. (2010). Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP. Proc. Natl. Acad. Sci. USA, 107: 17362-17367. PubMed Icon Google Scholar Icon

Wiese, M., Antebi, A. and Zheng, H. (2010). Intracellular Trafficking and Synaptic Function of APL-1 in Caenorhabditis elegans. PLoS ONE, 5: e12790. PubMed Icon Google Scholar Icon

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