Defining the Role of Tau and Kinase Regulator Chaperone Protein Cdc37 in Alzheimer’s Disease

Umesh Jinwal, PhD
University of South Florida (Tampa, FL)
Year Awarded:
2015
Grant Duration:
July 1, 2015 to June 30, 2017
Disease:
Alzheimer's Disease
Award Amount:
$200,000
Grant Reference ID:
A2015666S
Award Type:
Standard
Award Region:
US Southwestern
Umesh Jinwal, PhD

Investigating the Role of Cdc37 Co-Chaperone in Alzheimer’s Disease Pathogenesis

Summary

Abnormalities in tau protein have been linked to Alzheimer's disease (AD) and several other neurodegenerative diseases. These diseases are becoming more and more prevalent in our aging population. We propose to provide novel insight into tau abnormalities by investigating alterations of a chaperone protein, Cdc37, in cellular and animal models of AD. Chaperones are proteins that assist other proteins in achieving their final and functional shape, and play an important role in regulating protein synthesis and degradation of certain proteins such as tau. 

Details

The main focus of this research is to investigate the role of Cdc37, a chaperone protein, in tau abnormalities using cellular and animal models of AD. The goal is to determine how Cdc37 regulates aspects of tau pathogenesis and whether changing levels of Cdc37 in the brain might potentially decrease abnormalities in a well-characterized transgenic AD mouse model. We have previously shown that Cdc37 protein co-localizes with tau in neurons and physically interacts with tau in post-mortem brain tissues from AD patients. We have also shown that removal of Cdc37 from cells led to tau clearance, whereas excess Cdc37 in cells preserved tau. Additionally, we found that modulation of Cdc37 level alters levels of kinases, which are enzymes that modify proteins by a process called phosphorylation. Interestingly, abnormal phosphorylation of tau is a hallmark of AD. These data further support a novel role of Cdc37 in tau biology.
 
My colleagues and I have previously created a number of novel models using cells in culture to study the function of human tau. Using these unique models and tools, our research team will study the role of Cdc37 in maintaining or decreasing tau levels in cells at the molecular level. To better understand the function of Cdc37 in the brain, we will utilize a virus-based genetic modification technique to deplete the expression of Cdc37 in the brains of normal mice and transgenic AD mice. Brain tissues from these mice will then be analyzed for changes in levels of tau and kinases. Successful completion of this project will reveal how the removal of Cdc37 affects tau pathogenesis in vivo and could underscore the potential use of Cdc37 as a drug target for AD and other tau-related disorders.

About the Researcher

Umesh K. Jinwal is an assistant professor in the Department of Pharmaceutical Sciences, College of Pharmacy, Byrd Alzheimer’s Institute at the University of South Florida in Tampa, FL. He received his PhD in biotechnology from the Central Drug Research Institute, Barkatullah University, in Bhopal, India. He has postdoctoral experience in neuroscience, entomology, and cancer research.  Dr. Jinwal is actively involved in teaching and training PharmD and research students. Dr. Jinwal’s laboratory uses genetically modified human cells, C. elegans, and mouse models to study the complex mechanisms involved in Alzheimer’s disease and other neurodegenerative diseases. Dr. Jinwal’s ultimate goal is to discover new drugs for treating AD and other neurodegenerative diseases by understanding the molecular mechanism behind the pathogenesis of these diseases. He has applied for several patents; has published more than 49 manuscripts in peer-reviewed journals and 100 abstracts in scientific meetings; and his work has been cited over 1,000 times by researchers across the globe.


"The current absence of drugs to cure AD could be due to a poor understanding of the molecular mechanisms involved in AD progression, and the limited number of viable protein targets that potentially could be modified by drugs. Here, we propose to use a unique combination of in vitro and in vivo studies to understand the molecular mechanism of a chaperone protein, Cdc37, in tau and tau kinase regulation during AD pathogenesis. Successful completion of the proposed study may provide some evidence for the use of Cdc37 as therapeutic target for AD and other related disorders."

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