Gene Therapy for Alzheimer's Disease Using HSV1 Vectors

Alfred Geller, PhD
Children's Hospital (Boston, MA)
Year Awarded:
Grant Duration:
April 1, 1994 to March 31, 1995
Alzheimer's Disease
Award Amount:
Grant Reference ID:
Award Type:
Award Region:
US Northeastern

Gene Therapy for Alzheimer's Disease Using HSV1 Vectors



[Book Chapter] Lim, F., Hartley, D., Starr, P., Song, S., Lang, P., Yu, L., Wang, Y., and Geller, A.I. (1997) Use of defective Herpes-derived plasmid vectors. In: Recombinant gene expression protocols. Methods Molecular Biology Series, R.S. Tuan, ed., pp. 223-232.

Song, S., Wang, Y., Bak, S.Y., Lang, P., et al. (1997) A HSV-1 vector containing the rat tyrosine hydroxylase promoter enhances both long-term and cell type-specific expression in the midbrain. J. Neurochem. 68:1792-1803.  

Hartley, D.M., Neve, R.L., Bryan, J., Ullrey, D., et al. (1996) Expression of the calcium binding protein, parvalbumin, in cultured cortical neurons using a HSV-1 vector enhances NMDA neurotoxicity. Brain Res. Mol. Brain Res. 40:285-296.  

Goodman, L.J., Valverde, J., Lim, F., Geswind, M.D., et al. (1996) Regulated release and polarized localization of brain-derived neurotrophic factor (BDNF) in hippocampal neurons. Mol. Cell. Neurosci. 7:222-238.  

[Book Chapter] Geller, A.I. (1996) HSV-1 plasmid vectors for gene transfer into neurons. In: Neuronal regeneration, reorganization, and repair. F. Seil, ed., Advances in Neurology, Raven Press, N.Y. 72:143-148.

Gussoni, E., Wang, Y., Fraefel, C., Steinman, L., et al. (1996) FISH detection of introduced DNA in gene therapy protocols. Nature Biotech. 14:1012-1016.  

Fraefel, C., Song, S., Lim, F., Lang, P., et al. (1996) Helper virus-free transfer of HSV-1 plasmid vectors into neural cells. J. Virol. 70:7190-7197.  

Lim, F., Hartley, D., Starr, P., Lang, P., et al. (1996) Generation of high-titer defective HSV-1 vectors using an IE 2 deletion mutant and quantitative study of expression in cultured cortical cells. Biotechniques. 20(3):460-469.  

Oh, Y.J., Moffat, M., Wong, S., Ullrey, D., Geller, A.I. et al. (1996) A herpes simplex virus-1 one vector containing the rat tyrosine hydroxylase promoter directs cell-type specific expression of beta-galactosidase in cultured rat peripheral neurons. Moloch. Brain Res. 35:227-236.  

Starr, P., Lim, F., Grant, F., Trask, L., Lang, P., and Geller, A.I. (1996) Long-term persistence of defective HSV-1 vectors in the rat brain is demonstrated by re-activation of gene expression following superinfection with helper virus. Gene Therapy. 3:615-623.  

[Book Chapter] O'Malley, K.L. and Geller, A.I. (1996) Gene Delivery using HSV-1 plasmid vectors. In: Genetic Manipulation of the Nervous System, D.S. Latchman, ed., pp. 115-126. [Book Chapter] Lim, F., Starr, P., Song, S., Hartley, D. et al. (1995) Methods for using a defective herpes simplex virus (HSV-1) vector system. In: DNA Cloning 4, Mammalian Systems. The Practical Approach Series. D.M. Glover, and B.D. Hames, eds., Oxford Univ. Press, Oxford, pp. 263-283. Geller, A.I., Naegele, J., O'Malley, K., and During, M.J. (1995) Technical comment on 'Long-term behavioral recovery in parkinsonian rats by an HSV vector expressing tyrosine hydroxylase'. Science. 269:856-857.  

Geller, A.I., Freese, A., During, M.J., and O'Malley, K.L. (1995) A HSV-1 vector expressing tyrosine hydroxylase causes production and release of L-dopa from cultured rat striatal cells. J. Neurochem. 64:487-496.  

Smith, R. L., Geller, A.I., Escudero, K.W., and Wilcox, C.L. (1995) Long-term expression in sensory neurons in tissue culture from herpes simplex virus type 1 (HSV-1) promoters in a HSV-1 -derived vector. J. Virology. 69:4593-4599.  

[PMID:nd] Neuwelt, E.A., Pagel, M.A., Geller, A.I., and Muldoon, L.L. (1995) Gene replacement therapy in the central nervous system: Viral vector-mediated therapy of global neurodegenerative disease. Behavioral Brain Sci. 18:1-9.[link not available]

Geschwind, M.D., Kessler, J.A., Geller, A.I., and Federoff, H.J. (1994) Transfer of the nerve growth factor gene into cell lines and cultured neurons using a defective herpes simplex virus vector. Transfer of the NGF gene into cells by a HSV-1 vector. Mol. Brain Res. 24:327-335.  

During, M.J., Naegele, J., O'Malley, K., and Geller, A.I. (1994) Long-term behavioral recovery in Parkinsonian rats by an HSV-1 vector expressing tyrosine hydroxylase. Science. 266(5189):1399-1403.  

[PMID:nd] Geller, A.I., During, M.J., and Neve, R.L. (1994) A defective herpes simplex virus vector system for gene transfer into neural cells in the adult brain: Applications to gene therapy and neuronal physiology. Methods Neurosci. 21:443-461.[link not available]

Bahr, B.A., Neve, R.L., Sharp, J., Geller, A.I., and Lynch, G. (1994) Rapid and stable gene expression in hippocampal slice cultures from a defective HSV-1 vector. Brain Res. Mol. Brain Res. 26(1-2):277-285.  

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